86 Biomarkers of Inflammation in Neonatal Calves Supplemented with Condensed Solubles from Milk Permeate

Abstract Neonatal and weaning periods are the most challenging phases for enteric infections in dairy calves. While different approaches used to minimize calf health issues, neonatal diet remains core approach enhance pre- post-weaning animal performance. Previous studies have shown that bioactive compounds, including oligosaccharides present of calves, enhanced their growth performance post-weaning. However, effects supplementation during period impact on status remain be elucidated. Thus, we aimed evaluate blood biomarkers inflammation calves supplemented with condensed solubles from milk permeate (CS) (from birth 21 d) Twenty-four newborn Holstein (n = 12/group) housed individual hutches were a randomized complete block design study. Calves blocked by date sex. At 2-days old, randomly assigned into two groups 6 females males per group): control no supplement; or treatment 50 g/d CS. Both fed pasteurized waste preweaning period. 2.83 L 2×/d week 1 5, 1×/d 6, weaned at 7. each feeding 25 g CS was added treatment. Healthy scores recorded daily. Calf measurements samples collected weekly. Data analyzed using MIXED procedures SAS 9.4 repeated measures. Significant differences declared P < 0.05. Overall, body weight, fecal respiratory similar between During first 3 weeks age, group×sex×time interaction observed enzyme myeloperoxidase (MPO) expressed neutrophils. Female had decreased MPO concentrations relative female controls 3. Other (i.e., haptoglobin) consequent liver response albumin, paraoxonase) did not differ groups. Based these parameters, there evidence systemic days age. circulating is an indicative phagocytosis neutrophils resulting local inflammatory responses. The can associated lesser microbial infiltration gut. latter suggests stimulate commensal microbiota host, which serve as decoy receptors pathogenic microorganisms blocking infiltration). findings suggest molecules feed supplements such help immune